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SUMMARY:Developing Bragg Coherent Diffractive Imaging for Biological cryst
 als at the Advanced Photon Source
DTSTART:20141120T063000Z
DTEND:20141120T080000Z
DTSTAMP:20261017T021700Z
UID:indico-contribution-707@events.synchrotron.org.au
DESCRIPTION:Speakers: Connie Darmanin (La Trobe)\, Brian Abbey (ARC COE Ad
 vanced Molecular Imaging)\, Keith Nugent (ARC COE Advanced Molecular Imagi
 ng)\, Ross Harder (Advanced Photon Source)\, Victor Streltsov (CSIRO)\, He
 nry Kirkwood (La Trobe University)\, Nicholas Phillips (LTU/CXS)\, Hannah 
 Coughlan (La Trobe University)\n\nBragg Coherent Diffractive Imaging (BCDI
 ) of radiation hard crystals (e.g. lead\, gold) is now a relatively mature
  technique for characterising elastic strain fields at the nanoscale.  The
  technique relies upon the fact that coherent diffraction from a crystal p
 roduces a continuous intensity distribution around every Bragg peak\; if s
 ampled correctly\, this information can be used to reconstruct the crystal
 s three-dimensional complex density. The corresponding phase of the densit
 y is related to the elastic strain which provides information on the atomi
 c displacements within the crystal lattice (Pfeifer et al\, 2006).\nFor pr
 otein crystals the presence of strain and disorder within the crystal can 
 have a critical influence on the size of crystal that can be grown and the
  quality of diffraction data that can be collected. Hence\, our group has 
 recently been applying BCDI to the high-resolution characterisation of ela
 stic strains in micron-sized protein crystals. One of the major experiment
 al challenges with such samples is that they are highly radiation sensitiv
 e\; however the combination of cryogenic temperatures and photon counting 
 detectors offers a possible solution.\nHere we present the first BCDI resu
 lts collected from protein crystals of Lysozyme measuring ~3.5 microns in 
 diameter using the newly developed cryo-BCDI setup at beamline 34-ID-C at 
 the Advanced Photon Source. Our preliminary reconstructions from the BCDI 
 data are promising\, and pave the way for strain analysis of crystals comp
 osed of more complex proteins in future experiments.  \nPfeifer\, M. et al
 (2006).Nature\,442(7098)\,63–6.\n\nhttps://events.synchrotron.org.au/eve
 nt/3/contributions/707/
LOCATION:NCSS Exhibition Area
RELATED-TO:indico-event-3@events.synchrotron.org.au
URL:https://events.synchrotron.org.au/event/3/contributions/707/
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