Stream Leader: Anotine van Oijen
Srishti Dar - Tata Institute of Fundamental Research, Mumbai, India. Breaking–down Complexity: in vitro Reconstitution of Cytokinesis.
My graduate research with Dr. Thomas Pucadyil, at Indian Institute of Science Education and Research, Pune has led to the development of a novel assay system of supported membrane tubes that allows for quantitative fluorescence microscopy- based detection and analysis of protein and membrane dynamics (Dar et al., Nature Protocols, 2017). We have used this assay to tease apart the mechanism by which dynamin catalyzes membrane fission (Dar et al., Nature Cell Biology, 2015) and investigate the role of the membrane binding pleckstrin homology domain in the fission reaction (Dar and Pucadyil, MBoC, 2017). Presently, I am working as an INSPIRE faculty at the Tata Institute of Fundamental Research, Mumbai, India on an independent project on building an assay system of protein-encapsulated GUVs to understand actomyosin ring dynamics.